Previous research background

My previous research experience had focused on discovery and development of the cutting edge technologies for plant genetic engineering and tissue culture across economically important dicotyledonary and monocotyledonary crop species, and functional genomics for Phytophthora diseases in potato and soybean, including five-year experience at Monsanto Co.

Current research programs

Plant transformation science plays critical roles for biotechnology and functional genomics as the global market value of transgenic crops exceeded $4.7 billion in 2004, and the demand for efficient plant transformation methods for functional genomics increases as genome sequences for plant species become rapidly available. The technology of plant transformation is only moderately or marginally successful in many important cultivars of crops, which can be a major limiting factor for the biotechnological exploitation of economically important plant species and it can also impair the wider application of genomic science. Numerous technologies have been developed to improve plant transformation efficiency. However, the main restriction to the advancement of plant transformation science is that little is known about the molecular mechanisms that underlie the technologies required for plant transformation.  My current research interests at the ISRR are to address the following areas:

1) Develop novel and efficient/high throughput transformation systems for economically important plant and microbe species

2) Molecular mechanisms of plant/microbe transformation

3) Develop disease resistance and ornamental traits for horticulture crops and clonal forest via

genetic engineering.

EDUCATION

Ph.D. degree in Botany and Plant Pathology, Michigan State University, East Lansing, Michigan, U.S.A.

M.S. degree in Genetics and Plant Breeding, Paris XI University, Paris, France.

B.S. degree in Agronomy, Southwest Agricultural University, P.R. China.

WORK EXPERIENCE

2008 to present

Faculty, Scientist II (Supervisor), the Institute for Advanced Learning and Research, Danville, Virginia, USA.

Research areas: 1) Develop novel transformation technologies for economically important plant and microbe species, 2) Develop high throughput micropropagation systems for economically important ornamental species, 3) Improvement of disease resistance, flavor, and health benefits of strawberry through insertional mutagenesis, genomics, functional genomics, metabolic and molecular marker approaches, 4) Develop disease resistance and ornamental traits for high value crops via plant genetic engineering and 5) Biochemical and molecular mechanisms of in vitro plant oxidative stress in plant transformation using a model plant.

2003 to present

Adjunct Professor, Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA.

Research interests: 1) Genetic engineering of plants and 2) Genetic engineering of microorganisms.

2005 to present

Adjunct Professor, Department of Forestry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA.

Research interests: 1) Genetic engineering of clonal forest and 2) Artificial seed system.

2005 to 2008

Research Assistant Professor, the Institute for Advanced Learning and Research, Danville, Virginia, USA.

Research areas: 1) Develop novel high throughput transformation systems for economically important plant and microbe species, 2) Develop high throughput micropropagation system for economically important ornamental species, 3) Molecular mechanisms of plant/microbe transformation and 4) Develop disease resistance and ornamental traits for high value horticulture crops and clonal forest via genetic engineering.

2003 to 2005

Principal Investigator/Senior Scientist/Functional Genomic Team Leader, VBI, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA.

Research interests: 1) Genetic engineering of microorganisms---Development of high-throughput fungal-like pathogen transformation systems and gene expression systems, 2) Microbial functional genomics to understand the interaction between microbe and plants and 3) Phytophthora resistance in soybean.

1997 to 2002

Senior Scientist (1998 - 2002) / PostDoctoral Scientist Sept. (1997 - 1998), Monsanto Co., St. Louis, Missouri, USA.

Research area: 1) Discovery and development of the technologies for plant transformation across economically important dicotyledonary and monocotyledonary crop species and 2) functional genomics for Phytophthora diseases in potato.

1995 to 1997

Post Doctoral Research Associate, Department of Plant & Soil Sciences, Mississippi State University, Starkville, USA.

Research area: Genetic engineering of soybean: discovery and development of novel soybean regeneration and gene transformation systems for soybean.

PUBLICATIONS

Articles/Abstracts

Yinghui Dan, Aaron Baxter, Song Zhang, Christopher J. Pantazis, Richard E. Veilleux. 2010. Development of Efficient Plant Regeneration and Transformation System for Impatiens Using Agrobacterium tumefaciens and Multiple Bud Cultures as Explants. BMC Plant Biology 10: 165-

Yinghui Dan, Song Zhang,Liang Shi, Heng Zhong, Thomas Chenier, Timothy Hawkes, Eric Clarke^, Manuel Sainz. 2010. Anti-Oxidative Compounds Enhancing Plant Transformation. 12th World Congress of the International Association for Plant Biotechnology, June 6-11, 2010, St. Louis, Missouri USA.

Song Zhang, Liang Shi, Heng Zhong, Thomas Chenier, Timothy Hawkes, Eric Clarke, Manuel Sainz, Yinghui Dan. 2010. Biological roles of antioxidative compounds in Agrobacterium-mediated transformation of tomato cv. MicroTom. 12th World Congress of the International Association for Plant Biotechnology, June 6-11, 2010, St. Louis, Missouri USA.

Veilleux, RE, JJ Ruiz-Rojas, KP Mills, CM Davis, RC Lindsay, T Ferguson, SH Holt, C Pantazas, Yinghui Dan, and V Shulaev. 2010. Insertional mutants in diploid strawberry.

Plant and Animal Genome XVIII Conference, Jan. 9-13, San Diego, California, USA.

Holt, SH, JJ Ruiz-Rojas, Yinghui Dan, V Shulaev and RE Veilleux. 2010. Characterization of insertional mutants in Fragaria vesca. Plant and Animal Genome XVIII Conference, Jan. 9-13, San Diego, California, USA.

Yinghui Dan, Charles L. Armstrong, Jimmy Dong, Xiaorong Feng, Joyce E. Fry, Greg E. Keithly, Brian J. Martinell, Gail A. Roberts, Lori A. Smith, Lalaine Tan, David R. Duncan. 2009. Lipoic Acid----A Unique Plant Transformation Enhancer. In Vitro Cell Dev Biol.-Plant. 45(6):630-638.

Yinghui Dan, Pantazis Christopher. 2009. Hellebores Micropropagation. Plant Biology 2009, July 18-22, Honolulu, Hawaii, USA.

Rumen Conev, Lisa Lipsey, Jeffrey Miller, Frederic Duis, Linda Pinkham, John  Wise, Douglas Hensel, William McCalleb, Gregory Eaton, Richard Baker, Barry Flinn, Zhiwu Li, Yinghui Dan, M. Javed Iqbal, Kedong Da, Brian O’Neil and Jerzy Nowak. 2009. Strategies for Evaluation and Introduction of Ornamental Germplasm in Virginia. HortScience 44(3): 580-581. (Abstract).

Yinghui Dan. 2008. Biological Functions of Antioxidants in Plant Transformation. In Vitro Cell Dev Biol.-Plant 44 (3) 149-161.---Invited Review Article.

Yinghui Dan and Richard E. Veilleux. 2008. Asparagus. In: Compendium of Transgenic Crop Plants: Transgenic Vegetable Crops. C. Kole and T. C. Hall (eds.). Blackwell Publishing, Oxford, UK, pp 205-216.---Invited book chapter

Christopher Pantazis, Barry S. Flinn, Richard E. Veilleux, Jeremy Pattison, Craig Nessler, Vladimir Shulaev and Yinghui Dan. 2008. Strawberry Transformation Using Kanamycin Selection. In Vitro Cell Dev Biol.-Plant 44:S65 (Abstract).

Richard E. Veilleux, Teruko Oosumi, Aaron J. Baxter, Sarah H. Holt, Juan J. Ruiz-Rojas, Jeremy Pattison, Barry Flinn, Yinghui Dan, Craig Nessler, Vladimir Shulaev. 2007. Insertional mutagenesis in the diploid strawberry, Fragaria vesca. (Submitted to Acta Horticulturae and under review).

Yinghui Dan, Zhangjun Fei and Christophe Rothan. 2007. MicroTom---A New Model Plant for Genomics. Genes, Genomes and Genomics 1(2): 167-179.---Invited Review Article.

Yinghui Dan, Aaron Baxter, Richard E. Veilleux. 2007. Development of a New Regeneration and Transformation System for Impatiens. In Vitro Cell Dev Biol. 43:S27 (Abstract).

Yinghui Dan, R. E. Rothrock. 2007. Development of an Efficient Micropropagation System for Helleborus. In Vitro Cell Dev Biol. 43:S48 (Abstract).

Yinghui Dan. 2006. High Throughput Transformation and Micropropagation Opportunities for Crop Development. In 11th International Association for Plant Tissue Culture & Biotechnology Congress “Biotechnology and Sustainable Agriculture 2006 and Beyond”, Beijing, China, p.70 (abstract).

Yinghui Dan. 2006. A Novel Plant Transformation Technology---Lipoic Acid. In Vitro Cell Dev Biol. 42:18A (Abstract).

Yinghui Dan, C. L. Armstrong, J. Dong, X. Feng, J. E. Fry, G. E. Keithly, B. J. Martinell, K. A. Rayford, G. A. Roberts, C. Rommens, L. A. Smith, L. Tan, D. R. Duncan. 2006. A Novel Plant Transformation Enhancer. In Vitro Cell Dev Biol. 42:41-A (Abstract).

Yinghui Dan, Hua Yan, Tichafa Munyikwa, Jimmy Dong, Yanling Zhang, and Charles L. Armstrong. 2005. MicroTom---A High-Throughput Model Transformation System for Functional Genomics. Plant Cell Reports. Online First.

Yinghui Dan. 2004. Plant pathogen genomics and transformation. In Vitro Cell. Dev. Biol. 40: VII-A (Abstract).

Yinghui Dan, Ming Cheng, Jimmy Dong, David R. Duncan, Xiaorong Feng, Lori A. Julson, Greg E. Keithly, Kim A. Rayford, Gail A. Roberts, Caius Rommens, Nina Sidorova, Lalaine Tan, Charles L. Armstrong. Lipoic acid---A novel transformation enhancer. 2002. St. Louis Monsanto Publication, MSL-17631.

Yinghui Dan, Hua Yan, Tichafa Munyikwa, Jimmy Dong, Yanling Zhang, and Caius Rommens. 2002. A novel high throughput model transformation system for functional genomics. St. Louis Monsanto Publication, MSL-17630.

Yinghui Dan, Tishu Cai and Joyce Fry. 2002. A novel meristem regeneration for wheat. In Vitro Cell. Dev. Biol. 38: 114-A (Abstract).

Yinghui Dan, Hua Yan, Tichafa Munyikwa, Jimmy Dong, Bei Zhang, Linda K. Lahman, Caius Rommens.  2001. MicroTom---A Model Functional Genomic Assay. In Vitro Cell. Dev. Biol. 38: 22-A (Abstract).

Yinghui Dan, Jimmy Dong, Hua Yan, Tichafa Munyikwa, Yanling Zhang, Caius Rommens. 2001. MicroTom as A High Efficient Model Transformation System. 2001., 2001 ASA, CSSA, and SSA Meeting (Abstract).

Yinghui Dan, Jimmy Dong, Hua Yan, Tichafa Munyikwa, Yanling Zhang, and  Caius Rommens. 2001. A High Efficient Model Transformation System. In Vitro Cell. Dev. Biol.  37 (6): 854 (Abstract).

Yinghui Dan and Nancy A. Reichert. 1998. Organogenic                                               regeneration of soybean from hypocotyl explants. In Vitro Cell.                                         Dev. Biol. 34: 14-21

Yinghui Dan, L.L. Mcdougald, J.M. Tyler, N.A. Reichert. 1998. Organogenic regeneration of soybean from hypocotyl explants and progeny analyses. In Vitro Cell. Dev. Biol. 34 (3): 69-A. (Abstract)

L.L. Mcdougald, Yinghui Dan, N.A. Reichert. 1998. Biolistic-                                    mediated transformation and regeneration of soybean. In Vitro                                          Cell. Dev. Biol. 34 (3): 52-A. (Abstract)

Yinghui Dan and Nancy A. Reichert. 1997. Soybean regeneration from hypocotyl explant and transformation via particle bombardment. In Vitro Cell. Dev. Biol. 33:67A (Abstract).

Yinghui Dan and C.T. Stephens. 1997. DNA polymorphisms in somaclonal variants of Asparagus officinalis L., resistant to Fusarium oxysporum f. sp. asparagi. Plant Tissue Culture and Biotechnology 3 (2):89-96.

Yinghui Dan and C.T. Stephens. 1995. The development of asparagus  somaclones with high levels of resistance to Fusarium oxysporum f.sp. asparagi and F. proliferatum. Plant Dis. 79:923-927.

Yinghui Dan and C.T. Stephens. 1994. Regeneration of Plants from Protoplasts of Asparagus officinalis L., pp. 1-13. In: Biotechnology in Agriculture and Forestry 11."Plant protoplast and Genetic Engineering V". Y.P.S. Bajaj (ed). Springer-Verlag Berlin, Heidelberg, Germany.---Invited book chapter.

Yinghui Dan. 1994. Development of Fusarium Resistance of Asparagus via Tissue Culture And Gene Transformation. Ph.D.  thesis. Michigan State University, U.S.A..

Yinghui Dan and C.T. Stephens. 1991. Studies of Protoplast Culture Types and Plant Regeneration from Callus-derived Protoplasts of Asparagus officinalis L. cv. Lucullus 234. Plant Cell, Tissue and Organ Culture 27: 321-331

Yinghui Dan and C.T. Stephens. 1991. Studies of Protoplast Culture Types and Plant Regeneration from Callus-derived Protoplasts of Asparagus officinalis L. cv. Lucullus 234. In Vitro 27:117A (Abstract)

Yinghui Dan. 1986. The Test of Obtainment of Haploid of Antirrhinum majus L. by the Techniques: Irradiated Pollen and Culture in Vitro, M.S. thesis. Paris XI University, France.

Selected Patents

Yinghui Dan, Manuel B. Sainz, Heng Zhong, Liang Shi, 2010. Novel Plant Transformation Systems. United States Patent Application No. 12/800,895.

Yinghui Dan, Tichafa R.I. Munyikawa, Kimberly A. Rayford and Caius M.T. Rommens. 2004. Use of lipoic acid in plant culture media. US Patent Pub. No.: US 2004/0133938 A1.

Yinghui Dan et al. 2004. Transgenic Plants with Enhanced Agronomic Traits. US Patent Provisional Application 60/638,099.

Yinghui Dan and Joyce E. Fry. 2003. Novel method for the production of transgenic plants. US Patent Pub. No.: US 2003/0110531 A1.

Yinghui Dan and Nancy A. Reichert. 1999. Method for soybean transformation and regeneration utilizing  hypocotyl explants. U.S. Patent No.: 97022503.

SCIENTIFIC SOCIETY MEMBERSHIP

A member of the Society for In Vitro Biology (SIVB).

A member of American Society of Plant Biologists (ASPB).

A member of The International Association for Plant Tissue Culture and Biotechnology

(IAPTC&B).

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