Kedong Da, PhD
Senior Scientist
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Miscellaneous Information

Miscellaneous Information:

Research Interests:

Over the past 20 years, my primary research focus has been plant tissue culture commercialization, molecular plant breeding and molecular plant biology. The crop species I am interested in includes high value horticulture crops (hellebores, azalea, daylily, lily, orchid, rose, lisianthus, sinningia, amaryllis, begonia, petunia, iceland poppy, xsanthosoma, and fruit trees including grape, apple, pear, and peach ) and biofuel crops (Miscanthus, Arundo Donax). The breeding method we currently utilize involves DNA markers and in vitro mutation breeding. I am also interested in plant DNA methylation analysis through the use of methylation sensitive amplification polymorphism (MSAP).

Current Research:

  • Commercial tissue culture - The objective is to develop high throughput, cost effective tissue culture protocols to propagate high value horticulture and biofuel crops. We use both solid and liquid culture system in our research; somatic embryogenesis and bioreactors are my preference.
  • Plant in vitro breeding - We use in vitro mutation breeding systems (induced mutation breeding and ploidy breeding) to create new plant materials. DNA markers (RAPD, AFLP, MSAP) were used in early mutant selection and confirmation.
  • Molecular Biology - DNA methylation is believed to play an important role in plant stress biology; I am interested in DNA methylation changes in plant-beneficial endophyte interactions. This study is closely connected with organic agriculture by reduction of fertilizer application.




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Kedong Da, PhD - Publications

Selected Publications

Kim, S., Da, K. and Mei, C. 2012. An efficient system for high-quality large-scale micropropagation of Miscanthus x giganteus plants. In Vitro Cellular & Developmental Biology – Plant. 48(6):613-619

Da, K., Nowak, J.and Flinn, B. 2012. Potato cytosine methylation and gene expression changes induced by a beneficial bacterial endophyte, Burkholderia phytofirmans strain PsJN. Plant Physiol Biochem. 50(1):24-34.

Ni, X., D,a K.,Buntin, D., Cottrell, T. E., Tillman, P. G., Olson, D.M., Powell, R., Jr., Lee, R. D., Wilson, J. P.and Scully, B. T. 2010.  Impact of brown stink bug (Heteroptera: Pentatomidae) feeding on corn grain yield components and quality.  Journal of Economic Entomology. J. Econ. Entomol. 103(6): 2072-2079

Ni, X., Da, K., Buntin, D.and Brown S. 2008. Physiological basis of fall armyworm (Lepidoptera:Noctuidae) resistance in seedlings of maize inbred lines with varying levels of silk maysin. Florida Entomologist. 91(4):537-545

Da, K.,McCurdy, J., Ozias-Akins, P., May, L. and Chee, P. 2006. Progress toward the development of transgenic cotton cultivars adapted to Georgia environments.

Ni, X., Krakowsky, M. and Da, K. 2005.Evaluation of Corn Hybrids for Resistance to Insects.

Zang, Y., Zheng, W. and Da, K. 2004. Effect of different plant growth regulators on apple roo ting in vitro. J.  Shihezi Univ. 22(3):201-203

Zang,Y., Zheng, W. and Da, K. 2004. Progress on biochemical change during plant somatic embryogenesis. J Shandong Agri. Univ. 35(1):131-136.

Zang, Y., Zheng, W. and Da, K. 2004. Progress on apple leaf culture and regeneration. Biotechnology. (2):15-18.

Zang,Y., Zhang, S.and Da, K. 2004. Studies on leaf regeneration, micropropagation and pot plant production in trailing petunia (Petunia Hybrida). Acta Agriculturea Nuclestae Sinica. 18(1):18-21

Da, K.,Zhang, S., Zang, Y., Wu, L. and Shu, H. 2004. Morphological study of direct somatic embryogenesis from in vitro leaves of apple. Acta Agriculturea Nuclestae Sinica. 18(2):118-120

Da, K.,Zhang, S., Zang, Y., Li, H., Jiao, J. and Wu, L. 2003. Studies on mini-rose tissue culture and pot plant production. Plant tissue culture and virus free plant technology. Chinese Sci. and Tech. Press. Beijing, China. P75-78.

Da, K.and Zhang, S. 2003. Leaf culture, propagation and pot plant production in Lisianthus (Eustoma grandiflorum). J. Shandong Agric. Univ. 34(4):494-498

Zhang, S., Da,K. andCao, C. 2003. Agrobacterium tumefaciens-mediated transformation of Chinese chive (Allium tuberosum Rottle) Acta Horticulturae Sinica. 2003, 30(1): 39-42

Zhang, S., Da,K. andCao, C. 2003. Effects of antibiotics on plant regeneration on Chinese chive (Allium tuberosume Rottle). Acta Agriculturea Nucleatae Sinica. 2003, 17(2): 101-104

Zhang, S., Da,K. andCao, C. 2002. Efficient plant regeneration via root tip culture on Chinese chive (Allium tuberosume Rottle). Acta Horticulturae Sinica. 29(2): 141-144

Zhang, S., Da, K. andCao, C. 2002. Rapid micropropagation system via in vitro culture on Amaryllis vittata and its embryogenesis.  Acta Horticulturae Sinica. 29(3): 285-287

Da, K.and Zhang, S. 2002. Construction of CpTI gene super expression binary vector and transfer into apple. Proceeding of International Apple Symposium. J. Shandong Agric. Univ. (Suppl): 175-178

Da, K. and Zhang, S. 2002. Study on adventitious shoot regeneretion and micro-propagation from in vitro leaves of Begonia×Elatior.  J. Shandong Agric. Univ. 33(1): 93-95

Zhang, S., Da,K., Wei, Y. and Wen, F. 2002. Shoot regeneration of various genotypes in vitro and optimization of hormone concentration and combination in Chinese cabbage. J. Shandong Agric. Univ. 33(1): 7-13

Da, K.and Zhang, S. 2001. Soamtic embryogenesis from leaves of Begonia Elatior. Acta Horticulturae Sinica. 28(2): 180-181

Da, K.,Cui, D. and Zhang, S. 2001. Transformation of apple using supper expression cowpea trypsin inhibitor (CpTI) gene. Acta Horticulturae Sinica. 28 (1): 57-58

Da, K.,Zhang, S. and Li, Y. 2001. Wounding induced efficient direct somatic embryogenesis in apple leaves. Acta Agriculturea Nucleatae Sinica. 15(5): 290-293

Da, K.,Zhang, S. and Shu, H. 2001. Progress in apple embroidgenesis. International Symposium on Biotechnology Application in Horticultural Crops. China Agri. Sci. Press, Beijing, China. P43-47

Da, K.,Zhang, S., Li. Y. and Qi, Z. 1996. Direct somatic embryogenesis from in vitro leaves of apple. Acta Horticulturae Sinica. 23(3): 241-245

Da, K.,Zhang, S., Li, Y. and Shu, H. 1996. Somatic embryogenesis from petiole in apple.  Acta Agriculturea Nucleatae Sinica.  10(2): 75-78

Da,K.,Li, Y. and Shu, H. 1995. Callus induction and plant regeneration in apple.  Acta Agriculturea Nucleatae Sinica.  9(3): 139-142