Skillsets
Molecular biology techniques:
- Nucleic Acids: DNA/RNA isolation, PCR, RT-PCR, real-time PCR, PCR mutagenesis, RACE, cloning (TOPO, In-Fusion & Gateway), ligation, transformation, Southern analysis and DNA sequence analysis
- Protein: isolation, quantification (Bradford & BCA), concentration (acetone precipitation), SDS-PAGE, Western detection, ELISAs and myelin basic protein assay
- Experience with bacterial expression and radioactive enzyme assays
- Next generation sequencing
Plant tissue culture:
- Line maintenance, protoplast isolation, grafting (in vivo and in vitro), transformation (Agrobacterium & biolistic) and Agro-infiltration
- Agrobacterium transformation of tobacco, carrot, tomato, potato (diploid and tetraploid), lettuce and chicory. Inoculation of grasses with bacterial endophytes
- Experience with β-glucuronidase (histochemical & fluorimetrical assays) and with green fluorescent protein expression and quantification
Microbiology:
- Isolation of bacterial endophytes from plant material
- Antibiosis assays for anti-bacterial/fungal screening
- Disc and well diffusion assays for anti-bacterial/fungal screening of bacterial extracts
Bacterial endophyte research::
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IAA, ACC deaminase, phosphate solubilization, nitrogen fixation siderophore and biofilm assays
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In vitro growth promotion screening
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Growth promotion studies with numerous agronomic crops in the greenhouse, field (strawberry) and hydroponic systems (nutrient film and deep-water culture)
